5.13: Understand How Plasmids and Viruses can Act as Vectors, which take up Pieces of DNA, and then Insert this Recombinant DNA into Other Cells

RECOMBINANT DNA: DNA that is artificially formed as a result of the transfer of genetic material from one species to another

VECTOR: Vehicle that is used to deliver Recombinant DNA into a target cell

VECTORS

Diagram showing a Plasmid and Virus as Vectors

PLASMIDS: The same restriction enzyme is used to cut out gene of interest from DNA, and to cut open Plasmid vector to leave sticky ends on both As a result, these sticky ends of the gene and Plasmid overlap via complementary base pairing to be joined together by Ligase enzyme, ensuring gene is inserted in the correct orientation Recombinant DNA is formed and inserted into Bacterial cell Consequently, Bacteria will pass on Recombinant DNA to other Bacteria when it reproduces via binary fission VIRUS: The same restriction enzyme is used to cut out gene of interest from DNA, and to cut open Plasmid of Virus to leave sticky ends on both As a result, these sticky ends of the gene and Plasmid overlap via complementary base pairing to be joined together by Ligase enzyme, ensuring gene is inserted in the correct orientation Recombinant DNA is formed and inserted into Virus Consequently, Virus will carry Recombinant DNA to infect other organisms that it comes into contact with, inserting Recombinant DNA into cell