Sunday, June 25, 2017

5.6: Practical: Investigate the Role of Anaerobic Respiration by Yeast in Different Conditions

EQUATION FOR ANAEROBIC RESPIRATION (YEAST):



EXPERIMENT:

CARBON DIOXIDE PRODUCTION BY YEAST


Diagram showing Carbon Dioxide Production by Yeast

METHOD:

  • Mix boiling tube of Sugar solution with Yeast, covering it with a layer of Oil to prevent the entry of Oxygen; hence, ensuring aerobic respiration
  • Boiling tube is connected to a test tube of Limewater via capillary tube that is kept airtight by a bung
  • Boiling tube of Sugar solution with Yeast is placed in a Water bath of set temperature
  • Rate of Carbon Dioxide production is measured by number of bubbles produced in a fixed time in test tube of Limewater
  • Compare results obtained at different temperatures

RESULTS:

  • As temperature increases towards optimum, the rate of Carbon Dioxide production will increase
  • This is because the increase in temperature towards optimum will allow optimum enzyme activity for Yeast enzymes, hence increasing the rate of Anaerobic respiration to produce Carbon Dioxide (and Ethanol)
  • However, as temperature increases above optimum, enzymes involved in Anaerobic respiration will denature, therefore causing the rate of Carbon Dioxide production to decrease

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