Sunday, June 25, 2017

2.9: Practical: Investigate Food Samples for the Presence of Glucose, Starch, Protein and Fat

TEST FOR GLUCOSE:

TEST FOR GLUCOSE


Diagram Showing the Test for Glucose using Benedict’s Solution

METHOD:

  • Add a few drops of Benedict's solution into test tube of test solution
  • Heat test tube placed in a water bath set at 60 - 70 °C for 5 minutes
  • Remove test tube from water bath and observe colour change

RESULT:

  • If Glucose is present, solution will turn brick red
  • If Glucose is not present, solution will stay blue



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TEST FOR STARCH:

TEST FOR STARCH


Diagram Showing the Test for Starch using Iodine Solution

METHOD:

  • Use pipette to transfer sample solution into wells on tile
  • Add a few drops of Iodine solution and observe colour change

RESULT:

  • If Starch is present, solution will turn blue-black
  • If Starch is not present, solution will stay brown



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TEST FOR PROTEIN:

TEST FOR PROTEIN


Diagram showing the Test for Protein

METHOD:

  • Add a few drops of Biuret solution into test tube of sample solution
  • Observe colour change

RESULT:

  • If Protein is present, solution will turn violet
  • If Protein is not present, solution will stay blue



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TEST FOR FAT:

TEST FOR FAT


Diagram showing the Test for Fats

METHOD:

  • Test tube of sample solution is mixed with 2cm3 of Ethanol and 2cm3 of distilled water
  • Observe colour change

RESULT:

  • If Fat is present, a milky - white emulsion will form
  • If Fat is not present, solution will remain colourless

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